superscript ii rnase h reverse transcriptase kit (Thermo Fisher)

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Thermo Fisher superscript ii rnase h reverse transcriptase kit
Superscript Ii Rnase H Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+reverse+transcriptase+kit/Ribonuclease+A/pm40930097-44-32-39
Average 99 stars, based on 1 article reviews

superscript ii rnase h reverse transcriptase kit - by Bioz Stars, 2026-09

99/100 stars

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Synthesized:

Article Title: Designing a multi-epitope construct using immuno-informatic tools to prepare a messenger RNA vaccine against Rhipicephalus microplus ticks
Article Snippet: .. The cDNA was synthesized by superscript reverse transcriptase kit (Cat. No: 18064-022, Thermo Fisher Scientific) from the mRNA. ..

Article Title: Designing a multi-epitope construct using immuno-informatic tools to prepare a messenger RNA vaccine against Rhipicephalus microplus ticks
Article Snippet: The final purified mRNA was subsequently concentrated to a concentration of 77.2 ng/μL which was assessed using a Nanodrop spectrophotometer (Thermo Fisher Scientific). .. Then, cDNA was synthesized using a Superscript reverse transcriptase kit (Cat. No: 18064-022, Thermo Fisher Scientific) from the mRNA transcribed by T7ultra mMESSAGE mMACHINE and then purified by MEGAclear. ..

Reverse Transcription:

Article Title: Designing a multi-epitope construct using immuno-informatic tools to prepare a messenger RNA vaccine against Rhipicephalus microplus ticks
Article Snippet: .. The cDNA was synthesized by superscript reverse transcriptase kit (Cat. No: 18064-022, Thermo Fisher Scientific) from the mRNA. ..

Article Title: Clinical and laboratory characterization of cutaneous leishmaniasis in Chinese migrant workers returned from Iraq
Article Snippet: .. Total RNA was extracted from these lesion samples and reverse-transcribed to cDNA using SuperScript Reverse Transcriptase kit (Invitrogen, Carlsbad, California). ..

Article Title: FN1 from cancer-associated fibroblasts orchestrates pancreatic cancer metastasis via integrin-PI3K/AKT signaling
Article Snippet: Total RNA was extracted from cells or tissues using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions for detecting mRNA. .. Equal amounts of RNA were reverse transcribed into cDNA using the SuperScript Reverse Transcriptase Kit (Thermo Fisher Scientific). .. Total cDNA was amplified and analyzed in the Fast Real-time PCR-7500 system using SYBR Green PCR Master Mix (Thermo Fisher Scientific).

Article Title: Insights into the role of the N6-methyladenosine reader IGF2BP3 in the progression of oral squamous cell carcinoma and its connection to cell-cycle control
Article Snippet: The extraction of total RNA from transiently transfected cells was performed using Trizol Regent (Tiangen, Beijing, China). .. Subsequently, cDNA synthesis was carried out using a Superscript Reverse Transcriptase kit (Thermo, USA), following the directions provided by the manufacture. ..

Article Title: A Foxf1-Wnt-Nr2f1 cascade promotes atrial cardiomyocyte differentiation in zebrafish
Article Snippet: .. Whole embryo RNA was obtained from groups of 30 24 hpf embryos using TRIzol (Invitrogen, Cat # 15596026) and Purelink RNA Microkit (Invitrogen, Cat # 12183016). cDNA was generated using 1μg total RNA and the Superscript Reverse Transcriptase kit (Invitrogen, Cat # 18080-051). .. RT-qPCR for dkk1a and axin1 , was carried out using Power SYBR Green PCR Master Mix (Applied Biosystems, Cat # 4368706) in a BioRad CFX-96 PCR machine.

Article Title: Designing a multi-epitope construct using immuno-informatic tools to prepare a messenger RNA vaccine against Rhipicephalus microplus ticks
Article Snippet: The final purified mRNA was subsequently concentrated to a concentration of 77.2 ng/μL which was assessed using a Nanodrop spectrophotometer (Thermo Fisher Scientific). .. Then, cDNA was synthesized using a Superscript reverse transcriptase kit (Cat. No: 18064-022, Thermo Fisher Scientific) from the mRNA transcribed by T7ultra mMESSAGE mMACHINE and then purified by MEGAclear. ..

Article Title: Transcriptional regulators ensuring specific gene expression and decision-making at high TGFβ doses
Article Snippet: .. First, the SuperScript Reverse Transcriptase kit was used to generate cDNA according the manufactures instruction (#18064014; Thermo Fisher Scientific) using Olig(dT)-primer for mRNA specificity. .. Specific forward and reverse primers for target genes of interest were designed and purchased from IDT (SMAD2; FWD: 5′ GGGTTTTGAAGCCGTCTATCAGC 3′, REV: 5′ CCAACCACTGTAGAGGTCCATTC 3′, SMAD3; FWD: 5′ CATCGAGCCCCAGAGCAATA 3′, REV: 5′ GTAACTGGCTGCAGGTCCAA 3′, SNAI1; FWD: 5′ GCCTAGCGAGTGGTTCTTCT 3′, REV: 5′ TGCTGGAAGGTAAACTCTGGATT 3′, SNAI2; FWD: 5′ TTCAACGCCTCCAAAAAGCC 3′, REV: 5′ AGAGATACGGGGAAATAATCACTGT 3′, RUNX1; FWD: 5′ CGTGGTCCTACGATCAGTCC 3′, REV: 5′ GTCGGGTGCCGTTGAGA 3′, SKIL; FWD: 5′ GGCTGAATATGCAGGACAG 3′, REV: 5′ TGAGTTCATCTTGGAGTTCTTG 3′, SKI; FWD: 5′ TTCCGAAAAGGACAAGCCGT 3′, REV: 5′ ACAGCCCAGGCTCTTATTGG 3′, JUNB; FWD: 5′ CCTGGACGATCTGCACAAGA 3′, REV: 5′ GTAGCTGCTGAGGTTGGTGT 3′, JUN; FWD: 5′ CCTGGACGATCTGCACAAGA 3′, REV: 5′ GTAGCTGCTGAGGTTGGTGT 3′, KLF10; FWD: 5′ CGAGGACGCACACAGGAGAA 3′, REV: 5′ GTCACTCCTCATGAACCGCC 3′, ATF3; FWD: 5′ TCCATCACAAAAGCCGAGGTAG 3′, REV: 5′ CTGCAGGCACTCCGTCTTC 3′, SERPINE1: FWD: 5′ GGCTGACTTCACGAGTCTTTCA 3′, REV: 5′ ATGCGGGCTGAGACTATGACA 3′, FN1: FWD: 5′ GTGTGATCCCGTCGACCAAT 3′, REV: 5′ CGACAGGACCACTTGAGCTT 3′, SMAD7: FWD: 5′ ACCCGATGGATTTTCTCAAACC 3′, REV: 5′ GCCAGATAATTCGTTCCCCCT 3′, AXL: FWD: 5′ ACTCTGGGAGAGGGAGAGTT 3′, REV: 5′ GAGCCTCATGACGTTGGGAT 3′) and used at final concentrations of 240 nM.

other:

cDNA Synthesis:

Generated:

Purification:

Article Title: Designing a multi-epitope construct using immuno-informatic tools to prepare a messenger RNA vaccine against Rhipicephalus microplus ticks
Article Snippet: The final purified mRNA was subsequently concentrated to a concentration of 77.2 ng/μL which was assessed using a Nanodrop spectrophotometer (Thermo Fisher Scientific). .. Then, cDNA was synthesized using a Superscript reverse transcriptase kit (Cat. No: 18064-022, Thermo Fisher Scientific) from the mRNA transcribed by T7ultra mMESSAGE mMACHINE and then purified by MEGAclear. ..

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